Journal:

Article Title: The Effects of Polyethylene Glycol on Gene Expression of Developing White Spruce Somatic Embryos 1 [w]

doi: 10.1104/pp.015214

Figure Lengend Snippet: Quality control of microarray experiments

Article Snippet: The purified DNA was denatured in 50% (w/v) dimethyl sulfoxide and spotted in four replicates onto Corning microarray technology-gamma amino propyl silane aminosilane-coated glass microscope slides (Corning, NY) by a 417 Arrayer (Affymetrix, Woburn, MA).

Techniques: Microarray

Journal:

Article Title: CmeR Functions as a Pleiotropic Regulator and Is Required for Optimal Colonization of Campylobacter jejuni In Vivo ▿

doi: 10.1128/JB.01796-07

Figure Lengend Snippet: Key bacterial plasmids and strains used in this study

Article Snippet: C. jejuni NCTC 11168 microarray slides were purchased from MWG Biotech, Inc. (now Ocimum Biosolutions).

Techniques: Plasmid Preparation, Sequencing, Variant Assay

Journal:

Article Title: CmeR Functions as a Pleiotropic Regulator and Is Required for Optimal Colonization of Campylobacter jejuni In Vivo ▿

doi: 10.1128/JB.01796-07

Figure Lengend Snippet: Identification of the transcription unit of the Cj0369c-cmeR operon. (A) Genomic organization and sequence features of the Cj0369c-cmeR operon. ORFs are indicated by large arrows. The gene designations are indicated under the arrows. The bent arrows indicate the locations and orientations of the primers used in PCR for cloning the operon. Part of the intergenic sequence is shown above the ORFs. The putative ribosome-binding site is indicated by bold type. The inverted repeat sequences are underlined. The start codon (ATG) of Cj0369c is indicated by lowercase letters. The transcription start site of the Cj0369c-cmeR operon is indicated by an asterisk. The −10, −16, and −35 sequences are overlined. (B) Diagram of the two plasmid constructs used for mapping the promoter for cmeR. The ORFs correspond to those in panel A. (C) Immunoblot analysis of CmeR from various C. jejuni strains using anti-rCmeR antibodies. Lanes 1 to 4 contained whole-cell lysates from wild-type strain NCTC 11168, 11168ΔcmeR carrying pRY107 (vector control), 11168ΔcmeR carrying pRYC7, and 11168ΔcmeR carrying pRYC1, respectively.

Article Snippet: C. jejuni NCTC 11168 microarray slides were purchased from MWG Biotech, Inc. (now Ocimum Biosolutions).

Techniques: Sequencing, Clone Assay, Binding Assay, Plasmid Preparation, Construct, Western Blot

Journal:

Article Title: CmeR Functions as a Pleiotropic Regulator and Is Required for Optimal Colonization of Campylobacter jejuni In Vivo ▿

doi: 10.1128/JB.01796-07

Figure Lengend Snippet: Induction of Cj0561c by bile salts. C. jejuni 11168W7 carrying pMW561 (A) and 11168W7ΔcmeR carrying pMW561 (B) were grown in MH broth or MH broth supplemented with cholic acid (CA) or taurocholic acid (TCA). The promoter activity of Cj0561c was measured by the β-galactosidase assay. The bars and error bars indicate the means and standard deviations of triplicate measurements, respectively.

Article Snippet: C. jejuni NCTC 11168 microarray slides were purchased from MWG Biotech, Inc. (now Ocimum Biosolutions).

Techniques: Activity Assay

Journal:

Article Title: CmeR Functions as a Pleiotropic Regulator and Is Required for Optimal Colonization of Campylobacter jejuni In Vivo ▿

doi: 10.1128/JB.01796-07

Figure Lengend Snippet: Effect of the Cj0561c mutation on colonization of chickens by C. jejuni. The experiment included three groups of birds. The first two groups of birds were inoculated with 11168W7 and 11168W7Δ561 (A). The third group was infected with a 1:1 mixture of the two strains (B). Each symbol indicates the log number of CFU/g of feces for a single chicken. The horizontal bars indicate the means of groups at the indicated times. The detection limit of the plating method is about 100 CFU/g of feces. DPI, days postinoculation.

Article Snippet: C. jejuni NCTC 11168 microarray slides were purchased from MWG Biotech, Inc. (now Ocimum Biosolutions).

Techniques: Mutagenesis, Infection

Journal:

Article Title: CmeR Functions as a Pleiotropic Regulator and Is Required for Optimal Colonization of Campylobacter jejuni In Vivo ▿

doi: 10.1128/JB.01796-07

Figure Lengend Snippet: Inactivation of CmeR reduces C. jejuni colonization in chickens. The experiment included four groups of birds. The first three groups were inoculated with 11168W7, 11168W7ΔcmeR, and 11168W7ΔcmeR+ (A). The fourth group was inoculated with a 1:1 mixture of 11168W7 and 11168W7ΔcmeR (B). Each symbol indicates the log number of CFU/g of feces for a single chicken. The horizontal bars indicate the means of groups at the indicated times. The detection limit of the plating method is approximately 100 CFU/g of feces. DPI, days postinoculation.

Article Snippet: C. jejuni NCTC 11168 microarray slides were purchased from MWG Biotech, Inc. (now Ocimum Biosolutions).

Techniques: